Pairwise structural comparison of tiagabine analogs gives new insights into their protein binding modes
© Zdrazil et al.; licensee BioMed Central Ltd. 2013
Published: 22 March 2013
Tiagabine (Gabitril®) is a selective inhibitor of the human gamma-aminobutyric acid (GABA) transporter 1 (hGAT-1), a transport protein belonging to the family of neurotransmitter-sodium-symporters (NSS). It is a marketed drug, used for treatment of epilepsy. However, the molecular basis of protein-ligand interaction remains obscure due to the lack of a 3D structure of the target protein.
In order to identify activity-determining structural features of a series of tiagabine analogs taken from literature [1–3], we chose an approach combining traditional methods of molecular modeling with exhaustive sampling of docking poses, and a pairwise comparison of structural features and their respective bioactivity values.
We determined a common binding mode of tiagabine analogs, which is in nice agreement with literature . Further, we were able to trace back considerable differences in inhibitory activities to distinct molecular attributes of the analogs.
Our study revealed the molecular explanation for the importance of a polar linker region and thus paves the way for subsequent screening efforts in the search for novel GAT-1 inhibitors.
- Andersen KE, et al: The synthesis of novel GABA uptake inhibitors. 1. Elucidation of the structure-activity studies leading to the choice of (R)-1-[4,4-bis(3-methyl-2-thienyl)-3-butenyl]-3-piperidinecarboxylic acid (tiagabine) as an anticonvulsant drug candidate. J Med Chem. 1993, 36: 1716-1725. 10.1021/jm00064a005.View ArticleGoogle Scholar
- Knutsen LJ, et al: Synthesis of novel GABA uptake inhibitors. 3. Diaryloxime and diarylvinyl ether derivatives of nipecotic acid and guvacine as anticonvulsant agents. J Med Chem. 1999, 42: 3447-3462. 10.1021/jm981027k.View ArticleGoogle Scholar
- Andersen KE, et al: Synthesis of novel GABA uptake inhibitors. 4. Bioisosteric transformation and successive optimization of known GABA uptake inhibitors leading to a series of potent anticonvulsant drug candidates. J Med Chem. 1999, 42: 4281-4291. 10.1021/jm980492e.View ArticleGoogle Scholar
- Skovstrup , et al: Homology modelling of the GABA transporter and analysis of tiagabine binding. Chem Med Chem. 2010, 5: 986-1000.View ArticleGoogle Scholar
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